Anti-Mouse IgG ATTO 532 Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 532 was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
FLISA Dilution: >1:20,000, Fluorochrome Protein Value: 1.7, IF Microscopy Dilution: >1:5,000, Western Blot Dilution: >1:10,000
Application Notes:
Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 532 has been tested by dot blot and is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
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