Anti-Mouse IgG1 Alkaline Phosphatase Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. IgG1 chain
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
IgG1 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Form:
Liquid (sterile filtered)
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:5,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:500 - 1:2,500
Application Notes:
Anti-Mouse IgG1 Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.
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