Rabbit Anti-Mouse IgG F(ab)2 Antibody Biotin Conjugate, Rabbit Anti Mouse IgG Fab2 Biotin Conjugated Antibody, Rabbit Anti Mouse IgG Fab2 Fragment Antibody Biotin Conjugation
Format:
IgG
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
no cross-adsorbtion
Anti-Mouse IgG F(ab)2 Biotin antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab)2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under contro
Clonality:
Polyclonal
Concentration:
2.0 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Form:
Lyophilized
Formula:
10 mM NaPO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Thimerosal
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Anti-Mouse IgG F(ab)2 Biotin antibody has been tested by ELISA and dot blot and is assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
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