rabbit Anti-Mouse IgM (mu chain) Antibody Biotin Conjugated, rabbit Anti Mouse IgM mu chain Biotin Conjugated Antibody
Format:
IgG
Target Specificity:
IgM (µ)
Cross-Adsorption (MinX):
no cross-adsorbtion
Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight
Clonality:
Polyclonal
Concentration:
1.5 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reactivity was observed against other mouse heavy or light chain proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Anti-Mouse IgM Biotin Conjugated Antibody has been tested by ELISA and has been assayed against 1.0 ug of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitution concentration is suggested for this product.
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