Rabbit IgG anti-Mouse IgG (F(ab)2)-Biotin, MinX Hu
Biozol Catalog Number:
DNA-SEC-183319
Supplier Catalog Number:
SEC-183319
Alternative Catalog Number:
DNA-SEC-183319
Manufacturer:
dianova
Host:
Rabbit
Category:
Antikörper
Application:
ELISA,IHC,WB
Species Reactivity:
Mouse
Immunogen:
Mouse IgG F(ab)2 fragment
Conjugation:
Biotin
Alternative Names:
Rabbit Anti-Mouse IgG F(ab)2 Biotin Conjugated Antibody, Rabbit Anti Mouse IgG Fab2 Antibody Biotin Conjugate, Rabbit Anti Mouse IgG Fab2 Fragment Antibody Biotin conjugation
Format:
IgG
Target Specificity:
IgG (F(ab')2)
Cross-Adsorption (MinX):
Human
Anti-Mouse IgG F(ab)2 Biotin antibody generated in rabbit recognizes the dimeric Fab portion of the mouse IgG molecule. Mouse IgG F(ab)2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under contro
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c) or Human Serum Proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:87,000 of the reconstitution concentration is suggested for this product.
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