Anti-Rabbit IgG (H&L) generated in goat detects rabbit Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:10,000-1:100,000, Immunohistochemistry Dilution: 1:500-1:5,000, Western Blot Dilution: 1:5,000-1:40,000
Application Notes:
HRP Conjugated Anti-Rabbit IgG Secondary Antibody is ideal for Chemiluminescent Western Blotting, ELISA assays and immunohistochemistry, immunoperoxidase electron microscopy, as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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