Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
Conjugation:
Alk. Phos.
Alternative Names:
goat anti-Rabbit IgG Antibody Alkaline Phosphatase Conjugation, goat anti-Rabbit IgG Alk Phos Conjugated Antibody, goat anti-rabbit IgG heavy and light chain Antibody conjugated to Alkaline phosphatase
Format:
IgG
Target Specificity:
IgG (H+L)
Cross-Adsorption (MinX):
no cross-adsorbtion
Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in goat detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
Anti-Rabbit IgG (H&L) Antibody Alkaline Phosphatase conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Form:
Liquid (sterile filtered)
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,1% NaN3
Target:
Rabbit
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:3,000 - 1:25,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:1,000 - 1:4,000
Application Notes:
Anti-Rabbit IgG (H&L) alkaline phosphatase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunohistochemistry as well as other alkaline phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
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