goat anti-Rat IgG IgA IgM Antibody, Goat Anti-rat IgGAM
Format:
IgG
Target Specificity:
IgG+IgM+IgA (H+L)
Cross-Adsorption (MinX):
no cross-adsorbtion
Rat IgM antibody recognizes the mu chain of the Rat IgM. No reaction was observed against Rat IgA or Rat IgG. ELISA was used to confirm specificity at less than 1% cross reactivity against other rat heavy or light chain isotypes. Anti-Rat IgM antibody is
Clonality:
Polyclonal
Concentration:
2.0 mg/mL
Isotype:
Ig
Buffer:
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Purity:
Goat-Anti-Rat IgG IgA IgM Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat immunoglobulin subclasses and chain combinations.
Form:
Liquid (sterile filtered)
Formula:
125 mM Sodium Borate,75 mM NaCl,5 mM EDTA,pH 8,0,sterile filtered,0,01% NaN3
Target:
Rat
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Goat-Anti-Rat IgG IgA IgM antibody is suitable for use in various immunoassays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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