Rabbit Anti Rat IgM (mu chain) Antibody, Rabbit Anti-Rat IgM mu chain Antibody
Format:
IgG
Target Specificity:
IgM (µ)
Cross-Adsorption (MinX):
no cross-adsorbtion
Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed
Clonality:
Polyclonal
Concentration:
2.4 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgM and Rat Serum. No reaction was observed against other rat heavy or light chain proteins.
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Rat
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:50,000 - 1:250,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:5,000 - 1:25,000
Application Notes:
Anti-Rat IgM mu heavy chain antibody is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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