HRP Conjugated Secondary Antibody is designed for western blotting, ELISA and Immunohistochemistry. Horseradish peroxidase conjugated antibodies are ideal for antibody detection assays that require lot-to-lot consistency.
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target:
Sheep
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:5,000 - 1:22,000, Immunohistochemistry Dilution: 1:300 - 1:1,500, Western Blot Dilution: 1:500 - 1:3,000
Application Notes:
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
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