Anti-Sheep IgG generated in rabbit detects sheep Immunoglobulin G. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two an
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Anti-Sheep IgG HRP Conjugated Antibody Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target:
Sheep
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:30,000 - 1:70,000, Immunohistochemistry Dilution: 1:250 - 1:1,500, Western Blot Dilution: 1:1,000 - 1:10,000
Application Notes:
Anti-Sheep IgG Antibody Pre-Adsorbed conjugated to horseradish peroxidase is available in a variety of formats. HRP Anti-Sheep IgG Secondary Antibody conjugate is suitable for western blot or dot blot, ELISA and immunohistochemistry, immunoperoxidase electron microscopy and as well as other enzymatic assays requiring lot-to-lot consistency.
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