Rabbit IgG anti-Sheep IgG (Fc)-Alk. Phos., MinX Hu
Biozol Catalog Number:
DNA-SEC-183559
Supplier Catalog Number:
SEC-183559
Alternative Catalog Number:
DNA-SEC-183559
Manufacturer:
dianova
Host:
Rabbit
Category:
Antikörper
Application:
ELISA,IHC,WB
Species Reactivity:
Sheep
Immunogen:
Sheep IgG F(c) fragment
Conjugation:
Alk. Phos.
Alternative Names:
Rabbit Anti-Sheep IgG F(c) alkaline phosphatase Conjugated Antibody, Rabbit Anti-Sheep IgG Fc alkaline phosphatase Conjugated Antibody, Rabbit Anti-Sheep IgG Fc Fragment Antibody alk phos Conjugation
Format:
IgG
Target Specificity:
IgG (Fc)
Cross-Adsorption (MinX):
Human
Anti-Sheep IgG F(c) generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of sheep IgG and often
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab) or Human Serum Proteins..
Form:
Liquid (sterile filtered)
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,01% NaN3
Target:
Sheep
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:2,000 - 1:10,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:500 - 1:2,500
Application Notes:
This product has been assayed against 1.0 ug of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product.
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