Anti-Sheep IgG (H&L) generated in rabbit detects sheep Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the
Clonality:
Polyclonal
Concentration:
2.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Sheep IgG and Sheep Serum.
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Sheep
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:140,000, Immunohistochemistry Dilution: 1:500 - 1:3,000, Western Blot Dilution: 1:1,000 - 1:5,000
Application Notes:
Anti-Sheep IgG Biotin Conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
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