Donkey IgG anti-Sheep IgG (H+L)-Biotin, MinX none

Catalog Number: DNA-SEC-183577
Article Name: Donkey IgG anti-Sheep IgG (H+L)-Biotin, MinX none
Biozol Catalog Number: DNA-SEC-183577
Supplier Catalog Number: SEC-183577
Alternative Catalog Number: DNA-SEC-183577
Manufacturer: dianova
Host: Donkey
Category: Antikörper
Application: ELISA,IHC,WB
Species Reactivity: Sheep
Immunogen: Anti-Sheep IgG whole molecule was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Conjugation: Biotin
Alternative Names: Donkey Anti-Sheep IgG biotin Conjugated Antibody, Donkey Anti-Sheep IgG Antibody BAC Conjugation
Format: IgG
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Sheep IgG antibody generated in donkey detects specifically Sheep IgG. This secondary antibody anti-Sheep is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immun
Clonality: Polyclonal
Concentration: 2.0 mg/mL
Isotype: Ig
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity: Anti-Sheep IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Sheep IgG and Sheep Serum.
Form: Lyophilized
Formula: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target: Sheep
Antibody Type: Secondary Antibody
Application Dilute: ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes: Anti-Sheep IgG is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.