Anti-Swine IgG F(ab)2 Antibody generated in rabbit recognizes the dimeric Fab portion of the Swine IgG molecule. Swine IgG F(ab)2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled co
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(ab)2 and Swine Serum. No reaction was observed against Swine IgG F(c).
Form:
Liquid (sterile filtered)
Formula:
50 mM TrisHCl,150 mM NaCl,1 mM MgCl,0,1 mM ZnCl,50% (v/v) Glycerol,pH 8,0,sterile filtered,0,01% NaN3
Target:
Swine
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:1,000 - 1:3,000, Immunohistochemistry Dilution: 1:200 - 1:1,000, Western Blot Dilution: 1:500 - 1:2,000
Application Notes:
Anti-Swine IgG F(ab)2 alkaline phosphatase conjugated antibody has been tested by ELISA and is suitable for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user.
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