Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more general
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross-reactivity against other human heavy or light chain isotypes.
Form:
Liquid (sterile filtered)
Formula:
125 mM Sodium Borate,75 mM NaCl,5 mM EDTA,pH 8,0,sterile filtered,0,01% NaN3
Target:
Human
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:25,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Anti-Human IgG (kappa chain) Antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry assays requiring lot-to-lot consistency.
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