F(ab)2 Anti-Human lambda chain Antibody generated in goat detects lambda light chain from human. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized an
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Purity:
F(ab)2 Human lambda chain Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c). Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
Form:
Lyophilized
Formula:
125 mM Sodium Borate,75 mM NaCl,5 mM EDTA,pH 8,0,lyophilisate,0,01% Gentamicin
Target:
Human
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:10,000 - 1:50,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:1,000 - 1:5,000
Application Notes:
F(ab)2 Anti-Human lambda chain Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
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