F(ab)2 Anti-Human IgG (H&L) Antibody generated in donkey detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulin
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Human IgG, and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat, and Sheep Serum Proteins.
Form:
Liquid (sterile filtered)
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,0,01% NaN3
Target:
Human
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000, Immunohistochemistry Dilution: 1:1,000-1:5,000, Western Blot Dilution: 1:2,000-1:10,000
Application Notes:
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
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