Donkey F(ab)2 anti-Rabbit IgG (H+L)-FITC, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Catalog Number: DNA-SEC-183843
Article Name: Donkey F(ab)2 anti-Rabbit IgG (H+L)-FITC, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
Biozol Catalog Number: DNA-SEC-183843
Supplier Catalog Number: SEC-183843
Alternative Catalog Number: DNA-SEC-183843
Manufacturer: dianova
Host: Donkey
Category: Antikörper
Application: FLISA,FACS,IF
Species Reactivity: Rabbit
Immunogen: Rabbit IgG whole molecule
Conjugation: FITC
Alternative Names: Donkey F(ab)2 Anti-Rabbit IgG Fluorescein Conjugated Antibody, Donkey Fab2 Anti-Rabbit IgG Antibody FITC Conjugation
Format: F(ab')2
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep
F(ab)2 Anti-Rabbit IgG (H&L) Antibody generated in donkey detects rabbit IgG. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasm
Clonality: Polyclonal
Concentration: 1.0 mg/mL
Isotype: Ig
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Form: Lyophilized
Formula: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target: Rabbit
Antibody Type: Secondary Antibody
Application Dilute: FLISA Dilution: 1:10,000 - 1:50,000, Flow Cytometry Dilution: 1:500 - 1:2,500, Fluorochrome Protein Value: 4.5, IF Microscopy Dilution: 1:1,000 - 1:5,000
Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.