F(ab)2 Anti-Rat IgG F(ab)2 Antibody generated in goat detects Rat F(ab)2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B
Clonality:
Polyclonal
Concentration:
1.3 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Purity:
F(ab')2 Rat IgG F(ab')2 Antibody Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Form:
Liquid (sterile filtered)
Formula:
10 mM NaPO4,250 mM NaCl,pH 7,2,sterile filtered,Azide/BSA free
Target:
Rat
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
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