F(ab)2 Anti-Rat IgG F(ab)2 Fluorescein Antibody generated in goat detects Rat F(ab)2. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted
Clonality:
polyclonal
Concentration:
1.5 mg/mL
Isotype:
Ig
Buffer:
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(ab’)2 and Rat Serum. No reaction was observed against Rat IgG F(c), anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse and Human Serum Proteins.
Form:
Lyophilized
Formula:
10 mM NaPO4,250 mM NaCl,pH 7,2,lyophilisate,0.05% NaN3
Target:
Rat
Antibody Type:
Secondary Antibody
Application Dilute:
FLISA Dilution: 1:50 - 1:200, Flow Cytometry Dilution: 1:500 - 1:2,500, Fluorochrome Protein Value: 2.8, IF Microscopy Dilution: 1:50 - 1:200, Western Blot Dilution: 1:50 - 1:200
Application Notes:
F(ab')2 Anti-Rat IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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