Donkey Fab Anti-Goat IgG Biotin Conjugated Antibody, Donkey Fab Fragment Anti-Goat IgG Antibody Biotin Conjugation
Format:
Fab
Target Specificity:
IgG (H+L)
Cross-Adsorption (MinX):
no cross-adsorbtion
Fab Anti-Goat IgG Antibody generated in donkey detects goat IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a va
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Fab Anti-Goat IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target:
Goat
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:600,000, Fluorochrome Protein Value: 10-20, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:5,000 - 1:20,000
Application Notes:
Fab Anti-Goat IgG antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Specific conditions for reactivity should be optimized by the end user.
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