Rabbit Fab anti-Human IgG (H+L)-FITC, MinX none

Catalog Number: DNA-SEC-183953
Article Name: Rabbit Fab anti-Human IgG (H+L)-FITC, MinX none
Biozol Catalog Number: DNA-SEC-183953
Supplier Catalog Number: SEC-183953
Alternative Catalog Number: DNA-SEC-183953
Manufacturer: dianova
Host: Rabbit
Category: Antikörper
Application: FLISA,FACS,IF
Species Reactivity: Human
Immunogen: Human IgG whole molecule
Conjugation: FITC
Alternative Names: Rabbit Fab Anti-Human IgG Antibody Fluorescein Conjugation, Rabbit Fab Anti-Human IgG FITC Conjugated Antibody
Format: Fab
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Fab Anti-Human IgG (H&L) Fluorescein Antibody generated in rabbit detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immun
Clonality: Polyclonal
Concentration: 0.5 mg/mL
Isotype: Ig
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum.  No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Form: Lyophilized
Formula: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target: Human
Antibody Type: Secondary Antibody
Application Dilute: FLISA Dilution: 1:10,000 - 1:50,000, Flow Cytometry Dilution: 1:500 - 1:2,500, Fluorochrome Protein Value: 1.3, IF Microscopy Dilution: 1:1,000 - 1:5,000
Application Notes: Fab Anti-Human IgG (H&L) Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.