Rabbit Fab Anti-Mouse IgG Antibody Peroxidase Conjugation, Rabbit Fab Anti-Mouse IgG HRP Conjugated Antibody
Format:
Fab
Target Specificity:
IgG (H+L)
Cross-Adsorption (MinX):
no cross-adsorbtion
Fab Anti-Mouse IgG (H&L) Peroxidase Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies ar
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
Fab Anti-MOUSE IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target:
Mouse
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:62,000, Immunohistochemistry Dilution: 1:1,000 - 1:4,000, Western Blot Dilution: 1:2,000 - 1:10,000
Application Notes:
Fab Anti-MOUSE IgG (H&L) is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Specific conditions for reactivity should be optimized by the end user.
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