Rabbit Fab anti-Rat IgG (H+L)-FITC, MinX none

Catalog Number: DNA-SEC-183977
Article Name: Rabbit Fab anti-Rat IgG (H+L)-FITC, MinX none
Biozol Catalog Number: DNA-SEC-183977
Supplier Catalog Number: SEC-183977
Alternative Catalog Number: DNA-SEC-183977
Manufacturer: dianova
Host: Rabbit
Category: Antikörper
Application: FLISA,FACS,IF
Species Reactivity: Rat
Immunogen: Rat IgG whole molecule
Conjugation: FITC
Alternative Names: Rabbit Fab Anti-Rat IgG Antibody Fluorescein Conjugation, Rabbit Fab Anti-Rat IgG FITC Conjugated Antibody
Format: Fab
Target Specificity: IgG (H+L)
Cross-Adsorption (MinX): no cross-adsorbtion
Fab Anti-Rat IgG (H&L) Antibody generated in rabbit detects rat IgG. Representing approximately 75% of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells
Clonality: Polyclonal
Concentration: 0.5 mg/mL
Isotype: Ig
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Rabbit Serum.  No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Form: Lyophilized
Formula: 20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% NaN3
Target: Rat
Antibody Type: Secondary Antibody
Application Dilute: FLISA Dilution: 1:10,000 - 1:50,000, Flow Cytometry Dilution: 1:500 - 1:2,500, Fluorochrome Protein Value: 1.2, IF Microscopy Dilution: 1:1,000 - 1:5,000
Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.