Rabbit Fab Anti-Rat IgG Antibody Peroxidase Conjugation, Rabbit Fab Anti-Rat IgG HRP Conjugated Antibody
Format:
Fab
Target Specificity:
IgG (H+L)
Cross-Adsorption (MinX):
no cross-adsorbtion
Fab Anti-Rat IgG (H&L) Antibody generated in rabbit detects immunoglobulin g from rat, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75% of serum immunoglobulins, IgG
Clonality:
Polyclonal
Concentration:
1.0 mg/mL
Isotype:
Ig
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Purity:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Rabbit Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Form:
Lyophilized
Formula:
20 mM K3PO4,150 mM NaCl,pH 7,2,lyophilisate,0,01% Gentamicin
Target:
Rat
Antibody Type:
Secondary Antibody
Application Dilute:
ELISA Dilution: 1:10,000 - 1:40,000, Immunohistochemistry Dilution: 1:500 - 1:2,500, Western Blot Dilution: 1:1,000 - 1:4,000
Application Notes:
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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