Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit

Product Overview

• Article Name: Mouse ERK1/2(Extracellular Signal Regulated Kinase 1/2) ELISA Kit
• Biozol Catalog Number: EBT-ELK0019
• Supplier Catalog Number: ELK0019
• Alternative Catalog Number: EBT-ELK0019-96, EBT-ELK0019-48, EBT-ELK0019-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Mouse
• Alternative Names: ERK, ERK-2, ERK2, ERT1, MAPK2, NS13, P42MAPK, PRKM1, PRKM2, p38, p40, p41, p41mapk, p42-MAPK

Product Properties

• Concentration: 50 ng/mL
• Range: 0.78-50 ng/mL
• Sensitivity: 0.12 ng/mL
• Samples: tissue homogenates, cell lysates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Enzyme & Kinase,Apoptosis,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ERK1/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ERK1/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ERK1/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse ERK1/2 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK0019