Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit

Catalog Number: EBT-ELK0023
Article Name: Horse AOPP(Advanced Oxidation Protein Products) ELISA Kit
Biozol Catalog Number: EBT-ELK0023
Supplier Catalog Number: ELK0023
Alternative Catalog Number: EBT-ELK0023-96, EBT-ELK0023-48, EBT-ELK0023-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Equine
Alternative Names: AOPP
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 49.7 pg/mL
Samples: Serum, plasma and other biological fluids
Application Notes: Assay Type: Competitive Inhibition. Assay length: 2h. Research Area: Tumor immunity. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse AOPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse AOPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Horse AOPP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0023