DRP1, DLP1, DVLP, DYMPLE, HDYNIV, VPS1, Dynamin-related protein 1, Dnm1p/Vps1p-like protein, Dynamin family member proline-rich carboxyl-terminal domain less
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DNM1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DNM1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DNM1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat DNM1L in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0215
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