Human gABRa5(Gamma-Aminobutyric Acid A Receptor Alpha 5) ELISA Kit
Biozol Catalog Number:
EBT-ELK0409
Supplier Catalog Number:
ELK0409
Alternative Catalog Number:
EBT-ELK0409-96, EBT-ELK0409-48, EBT-ELK0409-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Human
Alternative Names:
GABA(A) receptor subunit alpha-5
Concentration:
10 ng/mL
Range:
0.16-10 ng/mL
Sensitivity:
0.061 ng/mL
Samples:
Tissue homogenates and other biological fluids.
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human gABRa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human gABRa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human gABRa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human gABRa5 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0409
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