Cattle alpha1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit

Catalog Number: EBT-ELK0530
Article Name: Cattle alpha1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
Biozol Catalog Number: EBT-ELK0530
Supplier Catalog Number: ELK0530
Alternative Catalog Number: EBT-ELK0530-96, EBT-ELK0530-48, EBT-ELK0530-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Bovine
Alternative Names: AMBP, UTI, HCP, EDC1, HI30, IATIL, ITILC, ITI, ITIL, Alpha 1 Microglobulin/Bikunin Precursor, Growth-inhibiting protein 19, Uristatin, Uronic-Acid-Rich Protein, Trypstatin
Concentration: 100 ng/mL
Range: 1.57-100 ng/mL
Sensitivity: 0.66 ng/mL
Samples: serum, plasma and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,Immune molecule,Kidney biomarker,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle alpha1M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle alpha1M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle alpha1M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Cattle alpha1M in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0530