Rat Hspa5(78 kDa glucose-regulated protein) ELISA Kit
Biozol Catalog Number:
EBT-ELK0677
Supplier Catalog Number:
ELK0677
Alternative Catalog Number:
EBT-ELK0677-96,EBT-ELK0677-48,EBT-ELK0677-96X5
Manufacturer:
ELK Biotechnology
Category:
Kits/Assays
Species Reactivity:
Rat
Alternative Names:
78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein, Endoplasmic reticulum chaperone BiP, BIP
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes:
Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Hspa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hspa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Hspa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat Hspa5 in the samples is then determined by comparing the OD of the samples to the standard curve
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