Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Product Overview

• Article Name: Human 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
• Biozol Catalog Number: EBT-ELK0694
• Supplier Catalog Number: ELK0694
• Alternative Catalog Number: EBT-ELK0694-96, EBT-ELK0694-48, EBT-ELK0694-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: 25 Hydroxy Vitamin D,

Product Properties

• Concentration: 200 ng/mL
• Range: 3.13-200 ng/mL
• Sensitivity: 1.45 ng/mL
• Samples: serum, plasma and other biological fluids

Application Notes

• Application Notes: Assay Type: Competitive Inhibition. Assay length: 2.5h. Research Area: Reproductive science,Genetic science,Nutrition metabolism,Bone metabolism,. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human 25-OH-D. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 25-OH-D. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK0694