Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit

Catalog Number: EBT-ELK0814
Article Name: Human Anti-LKM1(Anti-Liver Kidney Microsomal type 1 IgG) ELISA Kit
Biozol Catalog Number: EBT-ELK0814
Supplier Catalog Number: ELK0814
Alternative Catalog Number: EBT-ELK0814-96, EBT-ELK0814-48, EBT-ELK0814-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: ANTI-LKM1
Concentration: 300 U/mL
Range: 4.69-300 U/mL
Sensitivity: 1.66 U/mL
Samples: serum, plasma and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-LKM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-LKM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-LKM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Anti-LKM1 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0814