Mouse TYROBP(TYRO Protein Tyrosine Kinase Binding Protein) ELISA Kit

Catalog Number: EBT-ELK0826
Article Name: Mouse TYROBP(TYRO Protein Tyrosine Kinase Binding Protein) ELISA Kit
Biozol Catalog Number: EBT-ELK0826
Supplier Catalog Number: ELK0826
Alternative Catalog Number: EBT-ELK0826-96, EBT-ELK0826-48, EBT-ELK0826-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Mouse
Alternative Names: DAP12, KARAP, PLOSL, Killer Activating Receptor Associated Protein, DNAX-Activation Protein 12
Concentration: 20 ng/mL
Range: 0.32-20 ng/mL
Sensitivity: 0.17 ng/mL
UniProt: O54885
Samples: Tissue homogenates, cell lysates and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TYROBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TYROBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TYROBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse TYROBP in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0826