Human SCUBE3(Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit

Catalog Number: EBT-ELK0882
Article Name: Human SCUBE3(Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit
Biozol Catalog Number: EBT-ELK0882
Supplier Catalog Number: ELK0882
Alternative Catalog Number: EBT-ELK0882-96, EBT-ELK0882-48, EBT-ELK0882-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: SCUB1_HUMAN antibody, SCUBE 1 antibody, SCUBE1 antibody, Signal peptide, CUB and EGF-like domain-containing protein 1 antibody, Signal peptide, CUB domain, EGF like 1 antibody
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 0.065 ng/mL
UniProt: Q8IX30
Samples: Tissue homogenates, cell lysates and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cardiovascular. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCUBE3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCUBE3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCUBE3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human SCUBE3 in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0882