Human B2-glycoprotein 1 (beta2-GP1) antibody lgG ELISA Kit

Catalog Number: EBT-ELK0936
Article Name: Human B2-glycoprotein 1 (beta2-GP1) antibody lgG ELISA Kit
Biozol Catalog Number: EBT-ELK0936
Supplier Catalog Number: ELK0936
Alternative Catalog Number: EBT-ELK0936-96, EBT-ELK0936-48, EBT-ELK0936-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: APOH, BG, B2G1, B2GP1
Concentration: 10 ng/mL
Range: 0.16-10 ng/mL
Sensitivity: 0.06 ng/mL
Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human beta2-GP1 lgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human beta2-GP1 lgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human beta2-GP1 lgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human beta2-GP1 lgG in the samples is then determined by comparing the OD of the samples to the standard curve
ELK0936