Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit

Product Overview

• Article Name: Rat p-JNK(Phosphorylated c-Jun N-terminal kinases) ELISA Kit
• Biozol Catalog Number: EBT-ELK0960
• Supplier Catalog Number: ELK0960
• Alternative Catalog Number: EBT-ELK0960-96, EBT-ELK0960-48, EBT-ELK0960-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Rat
• Alternative Names: Bsk, BSK, CG5680, D-JNK, D-junk, DBSK/JNK, dJnk, dJNK, DJNK, DJNK/bsk, Dmel\CG5680, DmJNK, dNJNK, jnk, Jnk, JNK, jnk (bsk), JNK/SAPK, Junk, p-JNK, P-JNK, pJNK, SAPKa

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.148 ng/mL
• Samples: tissue homogenates, cell lysates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat p-JNK in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK0960