Human GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit

Product Overview

• Article Name: Human GROa/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit
• Biozol Catalog Number: EBT-ELK1024
• Supplier Catalog Number: ELK1024
• Alternative Catalog Number: EBT-ELK1024-96, EBT-ELK1024-48, EBT-ELK1024-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: CXCL1, GRO1, GROa, GRO-A, MGSA-A, NAP3, SCYB1, Chemokine C-X-C-Motif Ligand 1, Melanoma Growth Stimulating Activity Alpha, Fibroblast Secretory Protein, Neutrophil Activating Protein 3

Product Properties

• Concentration: 1000 pg/mL
• Range: 15.63-1000 pg/mL
• Sensitivity: 5.8 pg/mL
• UniProt: P09341
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Cytokine,Tumor immunity,Infection immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GROa/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human GROa/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

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