Mouse BAFF/CD257(B-Cell Activating Factor) ELISA Kit

Product Overview

• Article Name: Mouse BAFF/CD257(B-Cell Activating Factor) ELISA Kit
• Biozol Catalog Number: EBT-ELK10525
• Supplier Catalog Number: ELK10525
• Alternative Catalog Number: EBT-ELK10525-96, EBT-ELK10525-96X5, EBT-ELK10525-48
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Mouse
• Alternative Names: CD257, TNFSF13B, BLYS, TALL1, THANK, TNFSF20, ZTNF4, B lymphocyte stimulator, Tumor Necrosis Factor Ligand Superfamily Member 13B, Dendritic cell-derived TNF-like, BAFF

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.067 ng/mL
• UniProt: Q9WU72
• Samples: serum, plasma, tissue homogenates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,Infection immunity,Immune molecule,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BAFF/CD257. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BAFF/CD257. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BAFF/CD257, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse BAFF/CD257 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK10525