Pig IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit

Catalog Number: EBT-ELK10862
Article Name: Pig IGFBP7(Insulin Like Growth Factor Binding Protein 7) ELISA Kit
Biozol Catalog Number: EBT-ELK10862
Supplier Catalog Number: ELK10862
Alternative Catalog Number: EBT-ELK10862-96, EBT-ELK10862-96X5, EBT-ELK10862-48
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Porcine
Alternative Names: FSTL2, IGFBP-7v, MAC25, PSF, TAF, IGFBP-rP1, MAC25 protein, Prostacyclin-stimulating factor, PGI2-stimulating factor, Tumor-derived adhesion factor
Concentration: 100 ng/mL
Range: 1.56-100 ng/mL
Sensitivity: 0.47 ng/mL
Samples: Serum, plasma and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IGFBP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IGFBP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IGFBP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Pig IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve