Mouse p-cresol ELISA Kit

Catalog Number: EBT-ELK10942
Article Name: Mouse p-cresol ELISA Kit
Biozol Catalog Number: EBT-ELK10942
Supplier Catalog Number: ELK10942
Alternative Catalog Number: EBT-ELK10942-96, EBT-ELK10942-96X5, EBT-ELK10942-48
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Mouse
Alternative Names: 4-Hydroxytoluene, 4-Methylphenol, p-Cresol 98+ %, PARA-CRESOL, 4-cresol, para cresol
Concentration: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sensitivity: 3.6 pg/mL
Samples: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Neuro science,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-cresol. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-cresol. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-cresol, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse p-cresol in the samples is then determined by comparing the OD of the samples to the standard curve