Rat APOH(Apolipoprotein H) ELISA Kit

Catalog Number: EBT-ELK11132
Article Name: Rat APOH(Apolipoprotein H) ELISA Kit
Biozol Catalog Number: EBT-ELK11132
Supplier Catalog Number: ELK11132
Alternative Catalog Number: EBT-ELK11132-96, EBT-ELK11132-48, EBT-ELK11132-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Rat
Alternative Names: Apo-H, B2G1, BG, B2-GP1, B2GP1, Previously, Beta 2 Glycoprotein 1, APC inhibitor, Activated protein C-binding protein, Anticardiolipin cofactor, Beta(2)GPI
Concentration: 500 µg/mL
Range: 7.82-500 µg/mL
Sensitivity: 2.1 µg/mL
UniProt: P26644
Samples: Serum, plasma and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat APOH in the samples is then determined by comparing the OD of the samples to the standard curve
ELK11132