Rat E-Cad(E-Cadherin) ELISA Kit

Product Overview

• Article Name: Rat E-Cad(E-Cadherin) ELISA Kit
• Biozol Catalog Number: EBT-ELK11193
• Supplier Catalog Number: ELK11193
• Alternative Catalog Number: EBT-ELK11193-96, EBT-ELK11193-48, EBT-ELK11193-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Rat
• Alternative Names: CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule, Cadherin, Epithelial

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.13 ng/mL
• UniProt: Q9R0T4
• Samples: serum, plasma, urine, tissue homogenates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Rat E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK11193