Goat E-Cad(E-Cadherin) ELISA Kit

Catalog Number: EBT-ELK11194
Article Name: Goat E-Cad(E-Cadherin) ELISA Kit
Biozol Catalog Number: EBT-ELK11194
Supplier Catalog Number: ELK11194
Alternative Catalog Number: EBT-ELK11194-96, EBT-ELK11194-48, EBT-ELK11194-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Goat
Alternative Names: CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule, Cadherin, Epithelial
Concentration: 20 ng/mL
Range: 0.32-20 ng/mL
Sensitivity: 0.121 ng/mL
Samples: serum, plasma, urine, tissue homogenates and other biological fluids
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: CD & Adhesion molecule,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Goat E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve
ELK11194