Mouse IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit

Product Overview

• Article Name: Mouse IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
• Biozol Catalog Number: EBT-ELK1265
• Supplier Catalog Number: ELK1265
• Alternative Catalog Number: EBT-ELK1265-96, EBT-ELK1265-48, EBT-ELK1265-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Mouse
• Alternative Names: FABPI, IFABP, I-FABP, Intestinal-type fatty acid-binding protein, FABP2, Fatty Acid Binding Protein 2, Intestinal

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.055 ng/mL
• UniProt: P55050
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Metabolic pathway,Tumor immunity,Endocrinology,Cardiovascular biology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

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