Mouse NF-kappaB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit

Product Overview

• Article Name: Mouse NF-kappaB p105(Nuclear factor NF-kappa-B p105 subunit) ELISA Kit
• Biozol Catalog Number: EBT-ELK1337
• Supplier Catalog Number: ELK1337
• Alternative Catalog Number: EBT-ELK1337-96, EBT-ELK1337-48, EBT-ELK1337-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Mouse
• Alternative Names: NF-KB1, EBP-1, KBF1, NF-Kappa-B, NFKB-P105, NFKB-P50, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1, Nuclear factor NF-kappa-B p105 subunit, NFkB, Nuclear Factor Kappa B

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.058 ng/mL
• UniProt: P25799
• Samples: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Signal transduction,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-kappaBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-kappaBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NF-kappaBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Mouse NF-kappaBp105 in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK1337