Human NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit

Product Overview

• Article Name: Human NFkappaB-p65(Nuclear Factor Kappa B p65) ELISA Kit
• Biozol Catalog Number: EBT-ELK1692
• Supplier Catalog Number: ELK1692
• Alternative Catalog Number: EBT-ELK1692-96, EBT-ELK1692-48, EBT-ELK1692-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: NFKB3, p65, REL-A, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3, Transcription Factor P65, Nuclear factor NF-kappa-B p65 subunit, RELA, V-Rel Reticuloendotheliosis Viral Oncogene Homolog A

Product Properties

• Concentration: 10 ng/mL
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL
• UniProt: Q04206
• Samples: tissue homogenates, cell lysates and other biological fluids

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Apoptosis,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFkappaB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFkappaB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFkappaB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NFkappaB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve

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ELK1692