Human a2PI(Alpha-2-Plasmin Inhibitor) ELISA Kit

Catalog Number: EBT-ELK1919
Article Name: Human a2PI(Alpha-2-Plasmin Inhibitor) ELISA Kit
Biozol Catalog Number: EBT-ELK1919
Supplier Catalog Number: ELK1919
Alternative Catalog Number: EBT-ELK1919-96, EBT-ELK1919-48, EBT-ELK1919-96X5
Manufacturer: ELK Biotechnology
Category: Kits/Assays
Species Reactivity: Human
Alternative Names: SERPINF2, AAP, A2-PI, API, PLI, A2AP, Alpha 2 Antiplasmin, Serpin Peptidase Inhibitor Clade F Member 2, Pigment Epithelium Derived Factor
Concentration: 100 ng/mL
Range: 1.57-100 ng/mL
Sensitivity: 0.67 ng/mL
Samples: Serum, plasma and other biological fluids.
Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Hematology,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human a2PI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human a2PI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human a2PI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human a2PI in the samples is then determined by comparing the OD of the samples to the standard curve
ELK1919