Human NNE(Enolase, Non Neuronal) ELISA Kit

Product Overview

• Article Name: Human NNE(Enolase, Non Neuronal) ELISA Kit
• Biozol Catalog Number: EBT-ELK1988
• Supplier Catalog Number: ELK1988
• Alternative Catalog Number: EBT-ELK1988-96, EBT-ELK1988-48, EBT-ELK1988-96X5
• Manufacturer: ELK Biotechnology
• Category: Kits/Assays
• Species Reactivity: Human
• Alternative Names: ENO1, ENO1L1, MPB1, PPH, Alpha Enolase, Enolase 1, Phosphopyruvate hydratase, Plasminogen-binding protein, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding

Product Properties

• Concentration: 20 ng/mL
• Range: 0.32-20 ng/mL
• Sensitivity: 0.128 ng/mL
• UniProt: P06733
• Samples: Serum, plasma and other biological fluids.

Application Notes

• Application Notes: Assay Type: Sandwich. Assay length: 3.5h. Research Area: Enzyme & Kinase,Tumor immunity,. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human NNE in the samples is then determined by comparing the OD of the samples to the standard curve

Images

ELK1988